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In this paper we report how optical techniques were applied in the cylinder of an optically accessible engine equipped with latest-generation EURO V diesel engine head. The injection strategy with high percentage of EGR, characteristic of real engine operating point, was adopted. In particular, the combustion behavior at 1500 rpm\2 bar BMEP was investigated. Alternative diesel fuels were used. In particular, rapeseed methyl ester (RME) and gas to liquid (GTL) were selected as representative of 1st and 2nd generation alternative diesel fuel, respectively. Combustion analysis was carried out in the engine combustion chamber by means of visible digital imaging. These measurements helped to analyze the chemical and physical events occurring during the mixture preparation and the combustion development. Ultraviolet (UV) digital imaging was also performed and the presence of characteristic radical, like OH, in the various phases of combustion was detected as well.