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The composition ratio of saturated and unsaturated fatty acid methyl esters (FAME) is depended on feedstock. Three FAMEs: soybean (SME), palm (PME) and coconut oil (CME) methyl esters were mixed to make fuels which have different composition ratio. The ignitability of fuel which mainly consisted of unsaturated FAME was inferior. Power was slightly reduced with increasing of mixing ratio of CME; however exhaust gas emissions were improved because CME contained a lot of oxygen atoms. Fuel which was equal mixture SME and CME indicated almost the same ignition characteristic as that of PME because they have same composition ratio.

A new bio-fuel i.e. the cellulosic liquefaction fuel (CLF) was developed for diesel engines. CLF was made from woods by direct liquefaction process. When neat CLF was supplied to diesel engine, the compression ignition did not occur, so that blend of CLF and diesel fuel was used. The engine could be operated when the mixing ratio of CLF was up to 35 wt%. CO, HC and NOx emissions were almost the same as those of diesel fuel when the mixing ratio of CLF was less than 20 wt% whereas the thermal efficiency slightly decreases with increase in CLF mixing ratio.