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The world energy demand has witnessed uncertainties in two dimensions. The scarcity and depletion of conventional petroleum sources are causes of great concern worldwide. Combustion of fossil fuels has led to unprecedented rise in the global CO2 level, leading to global warming. Therefore, efforts are underway in several countries to search for suitable alternative fuels that are environment friendly. Vegetable oils of non-edible nature are such alternative fuels, which can form part of potential solution. Vegetable oils, due to their agricultural origin, are able to reduce CO2 emissions to the atmosphere along with import substitution of petroleum products In the present research, experiment were designed to study the effect of reducing Neem oil's high viscosity by increasing the fuel temperature and thereby its effect on combustion and emission characteristics of the engine.