Search Results

Controlling microbial growth and biofilm formation in spacecraft water-distribution systems is necessary to protect the health of the crew. Methods to decontaminate the water system in flight may be needed to support long-term missions. We evaluated the ability of iodine and ozone to kill attached bacteria and remove biofilms formed on stainless steel coupons. The biofilms were developed by placing the coupons in a manifold attached to the effluent line of a simulated spacecraft water-distribution system. After biofilms were established, the coupons were removed and placed in a treatment manifold in a separate water treatment system where they were exposed to the chemical treatments for various periods. Disinfection efficiency over time was measured by counting the bacteria that could be recovered from the coupons using a sonication and plate count technique. Scanning electron microscopy was also used to determine whether the treatments actually removed the biofilm.

Two simulated spacecraft water systems are being used to evaluate the effectiveness of iodine for controlling microbial contamination within such systems. An iodine concentration of about 2.0 mg/L is maintained in one system by passing ultrapure water through an iodinated ion exchange resin. Stainless steel coupons with electropolished and mechanically-polished sides are being used to monitor biofilm formation. Results after three years of operation show a single episode of significant bacterial growth in the iodinated system when the iodine level dropped to 1.9 mg/L. This growth was apparently controlled by replacing the iodinated ion exchange resin, thereby increasing the iodine level. The second batch of resin has remained effective in controlling microbial growth down to an iodine level of 1.0 mg/L. Scanning electron microscopy indicates that the iodine has impeded but may have not completely eliminated the formation of biofilm.

A Total Organic Carbon (TOC) Analyzer has been developed for a Life Sciences Risk Mitigation Flight Experiment to be conducted on Spacehab and the Russian space station, Mir. Initial launch is scheduled for December 1996 (flight STS-81). The analyzer will be tested on the Orbiter in the Spacehab module, including when the Orbiter is docked at the Mir space station. The analyzer is scheduled to be launched again in May 1997 (STS-84) when it will be transferred to Mir. During both flights the analyzer will measure the quality of recycled and ground-supplied potable water on the space station. Samples will be archived for later return to the ground, where they will be analyzed for comparison to in-flight results. Water test samples of known composition, brought up with the analyzer, also will be used to test its performance in microgravity. Ground-based analyses of duplicates of those test samples will be conducted concurrently with the in-flight analyses.