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Particulate number (PN) standards in the current ‘Euro 6’ European emissions standards pose a challenge for engine designers and calibrators during the warm-up phases of cold direct injection spark ignition (DISI) engines. To achieve catalyst light-off in the shortest time, engine strategies are often employed which inherently use more fuel to attain higher exhaust temperatures. This can lead to the generation of locally fuel-rich regions within the combustion chamber and the emission of particulates. This investigation analyses the combustion structures during the transient start-up phase of an optical DISI engine. High-speed, colour 9 kHz imaging was used to investigate five important operating points of an engine start-up strategy whilst simultaneously recording in-cylinder pressure.