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Particulate Matter (PM) emissions reduction is an imminent challenge for Direct Injection Spark Ignition (DISI) engine designers due to the introduction of Particulate Number (PN) standards in the proposed Euro 6 emissions legislation aimed at delivering the next phase of air quality improvements. An understanding of how the formation of combustion-derived nanoparticulates in engines is affected by the engine operating temperature is important for air quality improvement and will influence future engine design and control strategies. This investigation has examined the effect on combustion and PM formation when reducing the engine operating temperature to -7°C. A DISI single-cylinder optical research engine was modified to simulate a range of operating temperatures down to the proposed -7°C.