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Two crops of lettuce (Lactuca sativa cv. Waldmann's Green) were grown in the Regenerative Life Support Systems (RLSS) Test Bed at NASA's Johnson Space Center. The RLSS Test Bed is an atmospherically closed, controlled environment facility for the evaluation of regenerative life support systems using higher plants. The chamber encloses 10.6 m2 of growth area under cool-white fluorescent lamps. Lettuce was double seeded in 480 pots, each containing about 250 cm3 of calcined-clay substrate. Each pot was irrigated with half-strength Hoagland's nutrient solution at an average total applied amount of 2.5 and 1.8 liters pot-1, respectively, over each of the two 30-day crop tests. Average environmental and cultural conditions during both tests were 23°C air temperature, 72% relative humidity, 1000 ppm carbon dioxide (CO2), 16h light/8h dark photoperiod, and 356 μmol m-2s-1 photosynthetic photon flux.

Exploration Life Support (ELS) is a technology development project under the National Aeronautics and Space Administration's (NASA) Exploration Technology Development Program. The ELS Project's goal is to develop and mature a suite of Environmental Control and Life Support System (ECLSS) technologies for potential use on human spacecraft under development in support of U.S. Space Exploration Policy. Technology development is directed at three major vehicle projects within NASA's Constellation Program: the Orion Crew Exploration Vehicle (CEV), the Altair Lunar Lander and Lunar Surface Systems, including habitats and pressurized rovers. The ELS Project includes four technical elements: Atmosphere Revitalization Systems, Water Recovery Systems, Waste Management Systems and Habitation Engineering, and two cross cutting elements, Systems Integration, Modeling and Analysis, and Validation and Testing.

With the Preliminary Design Review (PDR) for the Orion Crew Exploration Vehicle planned to be completed in 2009, Exploration Life Support (ELS), a technology development project under the National Aeronautics and Space Administration's (NASA) Exploration Technology Development Program, is focusing its efforts on needs for human lunar missions. The ELS Project's goal is to develop and mature a suite of Environmental Control and Life Support System (ECLSS) technologies for potential use on human spacecraft under development in support of U.S. Space Exploration Policy. ELS technology development is directed at three major vehicle projects within NASA's Constellation Program (CxP): the Orion Crew Exploration Vehicle (CEV), the Altair Lunar Lander and Lunar Surface Systems, including habitats and pressurized rovers.

During the period July 2001 to March 2002, the performance of a water-jacketed high intensity discharge lamp of advanced design was evaluated within a lamp test stand at The University of Arizona (UA), Controlled Environment Agriculture Center (CEAC) in Tucson, Arizona. The lamps and test stand system were developed by Mr. Phil Sadler of Sadler Machine Company, Tempe, Arizona, and supported by a Space Act Agreement between NASA-Johnson Space Center (JSC) and UA. The purpose was for long term testing of the prototype lamp and demonstration of an improved procedure for use of water-jacketed lamps for plant production within the close confines of controlled environment facilities envisioned by NASA within Bioregenerative Life Support Systems. The lamp test stand consisted of six, 400 watt water-cooled, high pressure sodium HID lamps, mounted within a framework.