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The Internal Active Thermal Control System (IATCS) aboard the International Space Station (ISS) contains an aqueous, alkaline fluid (pH 9.5±0.5) that aids in maintaining a habitable environment for the crew. Because microbes have significant potential to cause disease, adverse effects on astronaut health, and microbe-induced corrosion, the presence of both bacteria and viruses within IATCS fluids is of concern. This study sought to detect and identify viral populations in IATCS samples obtained from the Kennedy Space Center as a first step towards characterizing and understanding potential risks associated with them. Samples were concentrated and viral nucleic acids (NA) extracted providing solutions containing 8.87-22.67 μg NA per mL of heat transfer fluid. After further amplification viral DNA and cDNA were then pooled, fluorescently labeled, and hybridized onto a Combimatrix panvira 12K microarray containing probes for ∼1,000 known human viruses.

The Moon Mineralogy Mapper (M3) instrument is scheduled for launch in 2008 onboard the Indian Chandrayaan-1 spacecraft. The mission is managed by the Indian Space Research Organization (ISRO) in Bangalore, India and is India's first flight to the Moon. M3 is being developed for NASA by the Jet Propulsion Laboratory under the Discovery Program Office managed by Marshall Space Flight Center. M3 is a state-of-the-art instrument designed to fulfill science and exploratory objectives. Its primary science objective is to characterize and map the lunar surface composition to better understand its geologic evolution. M3's primary exploration goal is to assess and map the Moon mineral resources at high spatial resolution to support future targeted missions. M3 is a cryogenic near infrared imaging spectrometer with spectral coverage of 0.4 to 3.0 μm at 10 nm resolution with high signal to noise ratio, spatial and spectral uniformity.

The Multi-angle Imaging SpectroRadiometer (MISR) instrument was launched aboard NASA’s Earth Observing System (EOS) Terra spacecraft on December 18, 1999. The overall mission design lifetime for the instrument is 6 years. The EOS Terra spacecraft was placed in a sun-synchronous near-circular polar orbit with an inclination of 98.3 degrees and a mean altitude of 705 km. The overall objective of MISR is to provide a means to study the ecology and climate of Earth through the acquisition of global multiangle imagery on the daylit side of Earth. MISR views the sunlit Earth simultaneously at nine widely spaced angles, collects global images with high spatial detail in four colors at every angle. The images acquired, once calibrated, provide accurate measurements of brightness, contrast and color of reflected sunlight.

The Mars Science Laboratory will be the next Martian mobility system that is scheduled to launch in the fall of 2011. The ChemCam Instrument is a part of the MSL science payload suite. It is innovative for planetary exploration in using a technique referred to as laser breakdown spectroscopy to determine the chemical composition of samples from distances of up to about 9 meters away. ChemCam is led by a team at the Los Alamos National Laboratory and the Centre d'Etude Spatiale des Rayonnements in Toulouse, France. The portion of ChemCam that is located inside the Rover, the ChemCam Body Unit contains the imaging charged-coupled device (CCD) detectors. Late in the design cycle, the ChemCam team explored alternate thermal design architectures to provide CCD operational overlap with the Rover's remote sensing instruments. This operational synergy is necessary to enable planning for subsequent laser firings and geological context.

The Moon Mineralogy Mapper (M3) instrument is one in a suite of twelve instruments which will fly onboard the Indian Chandrayaan-1 spacecraft scheduled for launch in 2008. Chandrayaan-1 is India's first mission to the Moon and is being managed by the Indian Space Research Organization (ISRO) in Bangalore, India. Chandrayaan-1 overall scientific objective is the photo-selenological and the chemical mapping of the Moon. The primary science objective of the M3 instrument is the characterization and mapping of the lunar surface composition in the context of its geologic evolution. Its primary exploration goal is to assess and map the Moon mineral resources at high spatial resolution to support future targeted missions. It is a “push-broom” near infrared (IR) imaging spectrometer with spectral coverage of 0.4 to 3.0 μm at 10 nm resolution with high signal to noise ratio, spatial and spectral uniformity.

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

Early development of concepts for space structures up to 1000 meters in size was initiated in the early 1960's and carried through the 1970's. The enabling technologies were self-deployables, on-orbit assembly, and on-orbit manufacturing. Because of the lack of interest due to the astronomical cost associated with advancing the on-orbit assembly and manufacturing technologies, only self-deployable concepts were subsequently pursued. However, for over 50 years, potential users of deployable antennas for radar, radiometers, planar arrays, VLBF and others, are still interested and constantly revising the requirements for larger and higher precision structures. This trend persists today. An excellent example of this trend is the current DARPA/SPO ISAT Program that applies self-deployable structures technology to a 300 meter long active planar array radar antenna. This ongoing program has created a rare opportunity for innovative advancement of state-of-the-art concepts.

As part of its integrated system test bed capability, NASA's Advanced Life Support Program has undertaken the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. This facility--the Advanced Life Support Human-Rated Test Facility (HRTF) is currently being built at the Johnson Space Center. The HRTF is comprised of a series of interconnected chambers with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system will consist of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated command and control functions. Currently, a portion of this multichamber facility has been constructed and is being outfitted with basic utilities and infrastructure.

We developed a non-destructive and non-contact method for measuring stress at the mid-plane of tempered glass plates that uses Bragg scattering from a pair of thermal gratings. These gratings are formed by 1064 nm beams from a seeded Nd:YAG laser and we measure the polarization state of light from a 532 nm beam that scatters from both these thermal gratings. The change in polarization of the doubly scattered light with separation between the two gratings allows measurement of the in-plane stress. Stress measurements are reported.

The 2001 Mars Odyssey spacecraft Martian Radiation Environment Experiment (MARIE) is a solid-state silicon telescope high-energy particle detector designed to measure galactic cosmic radiation (GCR) and solar particle events (SPEs) in the 20 – 500 MeV/nucleon energy range. In this paper we discuss the instrument design and focus on the observations and measurements of SPEs at Mars. These are the first-ever SPE measurements at Mars. The measurements are compared with the geostationary GOES satellite SPE measurements. We also discuss some of the current interplanetary particle propagation and diffusion theories and models. The MARIE SPE measurements are compared with these existing models.

Poly (vinyl chloride) (PVC) matrix membranes which incorporate the ionophore nonactin have been evaluated as cation exchange membranes for ammonium ion transport in an electrolytic cell configuration. Interest exists for the development of cation selective membranes for removal of low levels (<200ppm) of ammonium ions commonly found in recycled effluent streams in such diverse applications as expected in a Space Station and commercial fisheries. Ammonium ions are generated as a decomposition product of urea and over time build up in concentration, thus rendering the water unsuitable for human consumption. Nonactin is commonly used in a PVC matrix for ion-selective electrodes.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

Previous studies indicated evidence of opportunistic pathogens in samples obtained during missions to the International Space Station (ISS). This study utilized TaqMan quantitative PCR to determine specific gene abundance in potable and non-potable ISS waters. Probe and primer sets specific to the small subunit rRNA genes were designed and used to elucidate overall bacterial rRNA gene numbers. In addition, primer-probe sets specific for Burkholderia cepacia and Stenotrophomonas maltophilia were optimized and genes of these two opportunistic pathogens quantified in the pre- and post-flight drinking water as well as coolant waters. This Q-PCR approach supports findings of previous culture-based studies however; the culture based studies may have underestimated the microbial burden of ISS drinking water.

NASA's Advanced Life Support Program has included as part of its long-range planning the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. As the designated NASA Field Center responsible for integration and testing of advanced life support systems, Johnson Space Center has undertaken the development of such a facility--the Advanced Life Support Human-Rated Test Facility (HRTF). As conceived, the HRTF is an interconnected five-chamber facility with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system which sustains the crew consists of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated control and monitoring functions.

The Tropospheric Emission Spectrometer (TES), launched on NASA's Earth Observing System Aura spacecraft on July 15, 2004 has successfully completed over three years in space and has captured a number of important lessons. The instrument primary science objective is the investigation and quantification of global climate change. TES measures the three-dimensional distribution of ozone and its precursors in the lower atmosphere on a global scale. It is an infrared (IR) high resolution, imaging Fourier Transform Spectrometer (FTS) with a 3.3 to 15.4 μm spectral coverage required for space-based measurements to profile essentially all infrared-active molecules present in the Earth's lower atmosphere. The nominal on-orbit mission lifetime is 5 years. The Aura spacecraft flies in a sun-synchronous near-circular polar orbit with 1:38 pm ascending node.

Launched on NASA's Aura spacecraft on July 15, 2004, JPL's Tropospheric Emission Spectrometer (TES) has been operating successfully for over three years in space. TES is an infrared high resolution, imaging fourier transform spectrometer with spectral coverage of 3.3 to 15.4 μm to measure and profile essentially all infrared-active molecules present in the Earth's lower atmosphere. It measures the three-dimensional distribution of ozone and its precursors in the lower atmosphere on a global scale. The Aura spacecraft was successfully placed in a sun-synchronous near-circular polar orbit with a mean altitude of 705 km and 98.9 minute orbit period. The observatory is designed for a nominal 5 year mission lifetime. The instrument thermal design features include four temperature zones needed for efficient cryogenic staging to provide cooling at 65 K, 180 K, 230 K and 300 K.

Launched on India's Chandrayaan-1 spacecraft on October 22, 2008, JPL's Moon Mineralogy Mapper (M3) instrument has successfully completed over six months of operation in space. M3 is one in a suite of eleven instruments, six of which are foreign payloads, flying onboard the Indian spacecraft. Chandrayaan-1, managed by the Indian Space Research Organization (ISRO) in Bangalore, is India's first deep space mission. Chandrayaan-1 was launched on the upgraded version of India's Polar Satellite Launch Vehicle (PSLV-XL) from the Satish Dhawan Space Centre, SHAR, Sriharikota, India. The primary science objective of the M3 instrument is the characterization and mapping of the lunar surface composition in the context of its geologic evolution. Its primary exploration goal is to assess and map the Moon mineral resources at high spatial resolution to support future targeted missions.

Integrating physicochemical and biological technologies into a regenerative life support system is a complex technical challenge. NASA recognizes that the depth and breadth of the challenge warrants a comprehensive investigation. NASA is implementing several ground-based projects to look at different systems integration issues. The combined efforts of these activities will enable NASA to develop regenerative life support systems for human exploration of the solar system in the 21st century. This paper provides an overview of NASA's overall approach to ground testing of integrated regenerative life support systems.

The development of mid-IR semiconductor diode lasers based on type-II interband cascade structures is presented. How these diode lasers can be developed to meet the requirements in chemical sensing applications is discussed.

The microbial burdens of 69 cabin air samples collected in-flight aboard commercial airliners were assessed via culture-dependent and molecular-based microbial enumeration assays. Cabin air samples from each of four separate flights aboard two different carriers were collected via air-impingement. Microbial enumeration techniques targeting DNA, ATP, and endotoxin were employed to estimate total microbial burden. The total viable microbial population ranged from 0 to 3.6 × 104 cells per 100 liters of air, as assessed by the ATP-assay. When these same samples were plated on minimal medium, anywhere from 2 to 80% of the viable population was cultivable. Five of the 29 samples examined exhibited higher cultivable plate counts than ATP-derived viable counts, perhaps a consequence of the dormant nature (lower concentration of intracellular ATP) of cells inhabiting these air cabin samples.