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The Internal Active Thermal Control System (IATCS) aboard the International Space Station (ISS) contains an aqueous, alkaline fluid (pH 9.5±0.5) that aids in maintaining a habitable environment for the crew. Because microbes have significant potential to cause disease, adverse effects on astronaut health, and microbe-induced corrosion, the presence of both bacteria and viruses within IATCS fluids is of concern. This study sought to detect and identify viral populations in IATCS samples obtained from the Kennedy Space Center as a first step towards characterizing and understanding potential risks associated with them. Samples were concentrated and viral nucleic acids (NA) extracted providing solutions containing 8.87-22.67 μg NA per mL of heat transfer fluid. After further amplification viral DNA and cDNA were then pooled, fluorescently labeled, and hybridized onto a Combimatrix panvira 12K microarray containing probes for ∼1,000 known human viruses.

Spacecraft thermal control systems are essential to provide the necessary thermal environment for the crew and to ensure that the equipment functions adequately on space missions. The Ultralight Fabric Reflux Tube (UFRT) was developed by the Pacific Northwest National Laboratory as a lightweight radiator concept to be used on planetary surface-type missions (e.g., Moon, Mars). The UFRT consists of a thin-walled tube (acting as the fluid boundary), overwrapped with a low-mass ceramic fabric (acting as the primary pressure boundary). The tubes are placed in an array in the vertical position with the evaporators at the lower end. Heat is added to the evaporators, which vaporizes the working fluid. The vapor travels to the condenser end section and condenses on the inner wall of the thin-walled tube. The resulting latent heat is radiated to the environment. The fluid condensed on the tube wall is then returned to the evaporator by gravity.

The Orbiting Carbon Observatory (OCO) instrument is scheduled for launch onboard an Orbital Sciences Corporation LEOStar-2 architecture spacecraft in December 2008. The instrument will collect data to identify CO2 sources and sinks and quantify their seasonal variability. OCO observations will permit the collection of spatially resolved, high resolution spectroscopic observations of CO2 and O2 absorption in reflected sunlight over both continents and oceans. OCO has three bore-sighted, high resolution, grating spectrometers which share a common telescope with similar optics and electronics. A 0.765 μm channel will be used for O2 observations, while the weak and strong CO2 bands will be observed with 1.61 μm and 2.06 μm channels, respectively. The OCO spacecraft circular polar orbit will be sun-synchronous with an inclination of 98.2 degrees, mean altitude of 705 km and 98.9 minute orbit period.

For planetary exploration, new thermal insulation materials are needed to deal with unique environmental conditions presented to extravehicular activity (EVA). The thermal insulation material and system used in the existing space suit were specifically designed for low orbit environment. They are not adequate for low vacuum condition commonly found in planetary environments with a gas atmosphere. This study attempts to identify the types of lofty nonwoven thermal insulation materials and the construction parameters that yield the best performance for such application. Lofty nonwovens with different construction parameters are evaluated for their thermal conductivity performance. Three different types of fiber material: solid round fiber, hollow fiber, and grooved fiber, with various denier, needling intensity, and web density were evaluated.

A porous plate sublimator (based on an existing Lunar Module LM-209 design) is baselined as a heat rejection device for the X-38 vehicle due to its simplicity, reliability, and flight readiness. The sublimator is a passive device used for rejecting heat to the vacuum of space by sublimating water to obtain efficient heat rejection in excess of 1,000 Btu/lb of water. It is ideally suited for the X-38/CRV mission as it requires no active control, has no moving parts, has 100% water usage efficiency, and is a well-proven technology. Two sublimators have been built and tested for the X-38 program, one of which will fly on the NASA V-201 space flight demonstrator vehicle in 2001. The units satisfied all X-38 requirements with margin and have demonstrated excellent performance. Minor design changes were made to the LM-209 design for improved manufacturability and parts obsolescence.

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

As part of its integrated system test bed capability, NASA's Advanced Life Support Program has undertaken the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. This facility--the Advanced Life Support Human-Rated Test Facility (HRTF) is currently being built at the Johnson Space Center. The HRTF is comprised of a series of interconnected chambers with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system will consist of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated command and control functions. Currently, a portion of this multichamber facility has been constructed and is being outfitted with basic utilities and infrastructure.

Hamilton Sundstrand has developed a scalable evaporative heat rejection system called the Multi-Fluid Evaporator (MFE). It was designed to support the Orion Crew Module and to support future Constellation missions. The MFE would be used from Earth sea level conditions to the vacuum of space. This system combines the functions of the Space Shuttle flash evaporator and ammonia boiler into a single compact package with improved freeze-up protection. The heat exchanger core is designed so that radial flow of the evaporant provides increasing surface area to keep the back pressure low. The multiple layer construction of the core allows for efficient scale up to the desired heat rejection rate. A full-scale unit uses multiple core sections that, combined with a novel control scheme, manage the risk of freezing the heat exchanger cores. A four-core MFE prototype was built in 2007.

Hamilton Sundstrand is under contract with the NASA Johnson Space Center to develop a scalable, evaporative heat rejection system called the Multi-Fluid Evaporator (MFE). It is being designed to support the Orion Crew Module and to support future Constellation missions. A MFE would be used from Earth sea level conditions to the vacuum of space. The current Space Shuttle configuration utilizes an ammonia boiler and flash evaporator system to achieve cooling at all altitudes. With the MFE system, both functions are combined into a single compact package with significant weight reduction and improved freeze-up protection. The heat exchanger core is designed so that radial flow of the evaporant provides increasing cross-sectional area to keep the back pressure low. Its multiple layer construction allows for efficient scale up to the desired heat rejection rate.

In a crewed spacecraft environment, atmospheric carbon dioxide (CO2) and moisture control are crucial. Hamilton Sundstrand has developed a stable and efficient amine-based CO2 and water vapor sorbent, SA9T, that is well suited for use in a spacecraft environment. The sorbent is efficiently packaged in pressure-swing regenerable beds that are thermally linked to improve removal efficiency and minimize vehicle thermal loads. Flows are all controlled with a single spool valve. This technology has been baselined for the new Orion spacecraft. However, more data was needed on the operational characteristics of the package in a simulated spacecraft environment. A unit was therefore tested with simulated metabolic loads in a closed chamber at Johnson Space Center during the last third of 2006. Tests were run at a variety of cabin temperatures and with a range of operating conditions varying cycle time, vacuum pressure, air flow rate, and crew activity levels.

Poly (vinyl chloride) (PVC) matrix membranes which incorporate the ionophore nonactin have been evaluated as cation exchange membranes for ammonium ion transport in an electrolytic cell configuration. Interest exists for the development of cation selective membranes for removal of low levels (<200ppm) of ammonium ions commonly found in recycled effluent streams in such diverse applications as expected in a Space Station and commercial fisheries. Ammonium ions are generated as a decomposition product of urea and over time build up in concentration, thus rendering the water unsuitable for human consumption. Nonactin is commonly used in a PVC matrix for ion-selective electrodes.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

Previous studies indicated evidence of opportunistic pathogens in samples obtained during missions to the International Space Station (ISS). This study utilized TaqMan quantitative PCR to determine specific gene abundance in potable and non-potable ISS waters. Probe and primer sets specific to the small subunit rRNA genes were designed and used to elucidate overall bacterial rRNA gene numbers. In addition, primer-probe sets specific for Burkholderia cepacia and Stenotrophomonas maltophilia were optimized and genes of these two opportunistic pathogens quantified in the pre- and post-flight drinking water as well as coolant waters. This Q-PCR approach supports findings of previous culture-based studies however; the culture based studies may have underestimated the microbial burden of ISS drinking water.

NASA's Advanced Life Support Program has included as part of its long-range planning the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. As the designated NASA Field Center responsible for integration and testing of advanced life support systems, Johnson Space Center has undertaken the development of such a facility--the Advanced Life Support Human-Rated Test Facility (HRTF). As conceived, the HRTF is an interconnected five-chamber facility with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system which sustains the crew consists of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated control and monitoring functions.

Integrating physicochemical and biological technologies into a regenerative life support system is a complex technical challenge. NASA recognizes that the depth and breadth of the challenge warrants a comprehensive investigation. NASA is implementing several ground-based projects to look at different systems integration issues. The combined efforts of these activities will enable NASA to develop regenerative life support systems for human exploration of the solar system in the 21st century. This paper provides an overview of NASA's overall approach to ground testing of integrated regenerative life support systems.

The microbial burdens of 69 cabin air samples collected in-flight aboard commercial airliners were assessed via culture-dependent and molecular-based microbial enumeration assays. Cabin air samples from each of four separate flights aboard two different carriers were collected via air-impingement. Microbial enumeration techniques targeting DNA, ATP, and endotoxin were employed to estimate total microbial burden. The total viable microbial population ranged from 0 to 3.6 × 104 cells per 100 liters of air, as assessed by the ATP-assay. When these same samples were plated on minimal medium, anywhere from 2 to 80% of the viable population was cultivable. Five of the 29 samples examined exhibited higher cultivable plate counts than ATP-derived viable counts, perhaps a consequence of the dormant nature (lower concentration of intracellular ATP) of cells inhabiting these air cabin samples.

In January 2004, two Mars Exploration Rovers (MER) landed on the surface of Mars to begin their mission as robotic geologists. A year prior to these historic landings, both rovers and the spacecraft that delivered them to Mars, were completing a series of environmental tests in facilities at the Jet Propulsion Laboratory. This paper describes the test program undertaken to validate the thermal design and verify the workmanship integrity of both rovers and the spacecraft. The spacecraft, which contained the rover within the aeroshell, were tested in a 7.5 m diameter thermal vacuum chamber. Thermal balance was performed for the near earth (hot case) condition and for the near Mars (cold case) condition. A solar simulator was used to provide the solar boundary condition on the solar array. IR lamps were used to simulate the solar heat load on the aeroshell for the off-sun attitudes experienced by the spacecraft during its cruise to Mars.

An integrated water recovery system was operated for 91 days in support of the Lunar Mars Life Support Test Project (LMLSTP) Phase III test. The system combined both biological and physical-chemical processes to treat a combined wastewater stream consisting of waste hygiene water, urine, and humidity condensate. Biological processes were used for primary degradation of organic material as well as for nitrification of ammonium in the wastewater. Physical-chemical systems removed inorganic salts from the water and provided post-treatment. The integrated system provided potable water to the crew throughout the test. This paper describes the water recovery system and reviews the performance of the system during the test.

Certain Eubacteria enter a viable but nonculturable (VBNC) state upon encountering unfavorable environmental conditions. VBNC cells do not divide on conventional media yet remain viable and in some cases retain virulence. Here, we describe the VBNC state of two opportunistic pathogens previously isolated from ISS potable waters, Burkholderia cepacia and Stenotrophomonas maltophilia. Artificially inoculated microcosms were exposed to the biocidal agents copper (CuSO4) and iodine (I2) in an attempt to induce nonculturablility. Viability was assessed via fluorescent microscopy (direct viable count assay coupled with BacLight™ staining) and metabolic activity was monitored by quantifying both intracellular ATP and transcribed rRNA (reverse transcriptase quantitative PCR). Culturablility was lost in both B. cepacia and S. maltophilia within two days of exposure to copper or high concentrations of iodine (6 or 8 ppm).

This paper provides an overview of the IMMWPS Integrated Ground Test Program, completed at the NASA Johnson Space Center (JSC) during October and November 2001. The JSC Crew and Thermal Systems Division (CTSD) has developed the IMMWPS orbital flight experiment to test the feasibility of a microbe-based water purifier for use in zero-gravity conditions. The IMMWPS design utilizes a Microbial Processor Assembly (MPA) inoculated with facultative anaerobes to convert organic contaminants in wastewater to carbon dioxide and biomass. The primary purpose of the ground test program was to verify functional operations and procedures. A secondary objective was to provide initial ground data for later comparison to on-orbit performance. This paper provides a description of the overall test program, including the test article hardware and the test sequence performed to simulate the anticipated space flight test program. In addition, a summary of significant results from the testing is provided.