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To more effectively improve the work on a vehicle assembly line, it is desirable to have a method by which the degree of work load on each person can be evaluated quantitatively; enables us to decide the priority order of improvement; and calculates the improvement effect. We developed a quantitative evaluation method of work load by introducing a concept of physiological stress generated regardless of the type of muscles involved. Applying the burden borne by the body to the load evaluation of various assembly operations involved the problem of complex load measuring methods. We solved this problem by categorizing the load conditions for various assembly operations and converting each to a standard state of loads evaluated by experiments.