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Ethanol is regarded as the promising alternative fuel for gasoline to meet the strict low emission standard for spark ignition engines. In this study, the spray mixture formation process for different ethanol blended fuels, including E0 (gasoline), E85 (85% volume of ethanol and 15% volume of gasoline) and E100 (ethanol), has been evaluated using hole-type nozzle by the measurement of Laser Absorption Scattering (LAS) technique in a constant volume vessel. Based on the principle of LAS, the quantitative vapor and liquid phase distribution from different ethanol blended fuel can be obtained by the light extinction regime. Aiming to analyze the effect of mixture formation and evaporation for different components of blended fuel or pure gasoline and ethanol, the vapor distribution of gasoline was determined by using p-xylene, which had similar physical properties to gasoline, especially higher boiling temperature components, and higher absorption for ultraviolet.