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The effect of injection pressure ranging from 100 to 300MPa on the ignition, flame development and soot formation characteristics of biodiesel fuel spray using a common rail injection system for direct injection (D.I.) diesel engine was investigated. Experiments were carried out in a constant volume vessel under conditions similar to the real engine condition using a single hole nozzle. Biodiesel fuels from two sources namely; palm oil (BDFp) and cooked oil (BDFc) with the commercial JIS#2diesel fuel were utilized in this research. The OH chemiluminescence technique was used to determine the ignition and the lift-off length of the combusting flame. The natural luminosity technique was applied to study the flame development and the two color pyrometry was applied for the soot formation processes. Ignition delay decreased as the injection pressure progressed from 100 to 300MPa. This was as a result of the enhanced mixing achieved at higher injection pressures.