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Lignocellulosic biomass consists of (hemi-) cellulose and lignin. Accordingly, an integrated biorefinery will seek to valorize both streams into higher value fuels and chemicals. To this end, this study evaluated the overall combustion performance of both cellulose- and lignin derivatives, namely the high cetane number (CN) di-n-butyl ether (DnBE) and low CN anisole, respectively. Said compounds were blended both separately and together with EN590 diesel. Experiments were conducted in a single cylinder compression ignition engine, which has been optimized for improved combustion characteristics with respect to low emission levels and at the same time high fuel efficiency. The selected operating conditions have been adopted from previous “Tailor-Made Fuels from Biomass (TMFB)” work.