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Biofuel usage is increasingly expanding thanks to its significant contribution to a well-to-wheel (WTW) reduction of greenhouse gas (GHG) emissions. In addition, stringent emission standards make mandatory the use of Diesel Particulate Filter (DPF) for the particulate emissions control. The different physical properties and chemical composition of biofuels impact the overall engine behaviour. In particular, the PM emissions and the related DPF regeneration strategy are clearly affected by biofuel usage due mainly to its higher oxygen content and lower low heating value (LHV). More specifically, the PM emissions and the related DPF regeneration strategy are clearly affected by biofuel usage due mainly to its higher oxygen content and lower low heating value, respectively. The particle emissions, in fact, are lower mainly because of the higher oxygen content. Subsequently less frequent regenerations are required.