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Microbial control in closed environmental systems, such as those of spacecraft or proposed base missions is typically limited to disinfection in the potable water system by a strong chemical agent such as iodine or chlorine. However, biofilm growth in the environmental system tubing threatens both the sterility of the potable water distribution as well as operational problems with wastewater systems. In terrestrial systems, biofilm has been recognized for its difficulty to control and eliminate as well as resulting operational problems. In order to maintain a potable water source for crew members as well as preventing operational problems in non-sterile systems, biofilm needs to be considered during system design. While biofilm controls can limit biofilm buildup, they are typically disruptive and cannot completely eliminate biofilm. Selenium coatings have shown to prevent initial biofilm attachment as well as limit attached growth on a variety of materials.

Simulated wastewater, known as early surface mission wastewater, treated in previous experiments at JSC and TTU included urinal flush water, shower water, humidity condensate, oral hygiene water, and hand wash water. In reality, there is a difference between the early surface mission wastewater and the International Space Station wastewater. The ISS does not have a shower or hand wash, which contributes approximately 59 percent of the make-up water treated. The average influent ammonia concentration in the simulated wastewater treated by the TTU water reclamation system frequently exceeds 500 mg/L. Removal of the shower make-up water in simulated wastewater will result in a significant increase in the ammonia concentration, resulting in higher influent pH values and ammonia concentrations that may be inhibitory. Biological treatment technologies have suitably treated the diluted waste stream but a more concentrated waste stream may present a greater challenge.