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Biodiesel is a potential substitute for diesel and extensive research is carried in India on production and utilization of biodiesel from a variety of edible/non-edible, animal fat and waste oils. However, issues like stability, clogging, increased NOx, and high consumption rate etc. are some of the critical issues which are associated with long-term use of these alternative fuels in a diesel engine. The recent developments in science and technology may have concreted a method to create nano measure vigorous resources that have incredible benefits to micron sized constituents. Nano liquids may be a fresh period of compact-fluid complex constituents comprising of nano sized concrete elements disseminated into a base liquid. The present study investigates the effect of doping metal oxides nanoparticles with waste fish oil-based biodiesel. For the present study, the blends of fuel are prepared by using 30ppm each of titanium dioxide and alumina nanoparticles respectively.