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A novel non-destroy repeatable-use impact theory based total cylinder sampling system has been established. This system is mainly composed of a knocking body and a sampling valve. The knocking body impacts the sampling valve with certain velocity resulting in huge force to open the sampling valve and most of the in-cylinder gas has been dumped to one sampling bag for after-treatment. The feasibility and sampling response characteristics of this impact theory based total cylinder sampling system were investigated by engine bench testing. Within 0 to 35°CA ATDC (Crank Angle After Top Dead Center) sample timing 50 percent to 80 percent of in-cylinder mass would be sampled, which was a little less compared with the traditional system. The half decay period of pressure drop was 10 to 20 degrees crank angle within 0 to 60°CA ATDC sample timing, which was about 2-3 times of the traditional system.