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Methyl esters derived from vegetable oils by the process of transesterification (commonly referred as ‘biodiesel’), can be used as an alternative fuel in compression ignition engines. In this study, three different vegetable oils (rape, soy and waste oil) were used to produce biodiesel fuels that were then tested in a four cylinder direct injection engine, typically used in small diesel genset applications. Engine performance and emissions were recorded at five load conditions and at two different speeds. This paper presents the results obtained for measurements of NOx and smoke opacity at the different speed and load conditions for the three biodiesels, and their blends (5 and 50% v/v) with mineral diesel. A simple combustion analysis was also performed where ignition delay, position and magnitude of peak cylinder pressure and heat release rate were examined to asses how the variation of chemical structure and blend percentage affects engine performance.