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A certification diesel fuel and blends containing 10 and 15 volume % ethanol were tested in a 5.9-liter Cummins B Series engine. For each fuel blend, an 8-mode AVL test cycle was performed. The resulting emissions were characterized and measured for each individual test mode (prescribed combination of engine speed and load). These individual mode results are used to create a weighted average that is designed to approximate the results of the Heavy-Duty Transient Federal Test Procedure. The addition of ethanol was observed to have no noticeable effect on the emission of NOx but produced small increases in CO and HC. However, the particulate matter was observed to decrease 20% and 30% with the addition of 10% and 15% ethanol, respectively.

Saab Automobile recently released the BioPower engines, advertised to use increased turbocharger boost and spark advance on ethanol fuel to enhance performance. Specifications for the 2.0 liter turbocharged engine in the Saab 9-5 Biopower 2.0t report 150 hp (112 kW) on gasoline and a 20% increase to 180 hp (134 kW) on E85 (nominally 85% ethanol, 15% gasoline). While FFVs sold in the U.S. must be emissions certified on Federal Certification Gasoline as well as on E85, the European regulations only require certification on gasoline. Owing to renewed and growing interest in increased ethanol utilization in the U.S., a European-specification 2007 Saab 9-5 Biopower 2.0t was acquired by the Department of Energy and Oak Ridge National Laboratory (ORNL) for benchmark evaluations. Results show that the vehicle's gasoline equivalent fuel economy on the Federal Test Procedure (FTP) and the Highway Fuel Economy Test (HFET) are on par with similar U.S.-legal flex-fuel vehicles.