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At the collision moment, a driver’s lower extremity will be in different foot position, which leads to the different posture of the lower extremity with various muscle activations. These will affect the driver’s injury during collision, so it is necessary to investigate further. A simulated collision scene was constructed, and 20 participants (10 male and 10 female) were recruited for the test in a driving simulator. The braking posture and muscle activation of eight major muscles of driver’s lower extremity (both legs) were measured. The muscle activations in different postures were then analyzed. At the collision moment, the right leg was possible to be on the brake (male, 40%; female, 45%), in the air (male, 27.5%; female, 37.5%) or even on the accelerator (male, 25%; female, 12.5%). The left leg was on the floor all along.