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Increasing diagnosis capabilities in modern engine electronic control units (ECUs), especially in the exhaust path, in terms of emission and engine aftertreatment control utilize on-board NOx prediction models. Nowadays it is an established approach at hardware-in-theloop (HIL) test benches to replicate the engine's steady-state NOx emissions on the basis of stationary engine data. However, this method might be unsuitable for internal ECU plausibility checks and ECU test conditions based on dynamic engine operations. Examples of proven methods for modeling the engine behavior in HIL system applications are so-called mean value engine models (MVEMs) and crank-angle-synchronous (in-cylinder) models. Of these two, only the in-cylinder model replicates the engine’s inner combustion process at each time step and can therefore be used for chemical-based emission simulation, because the formation of the relevant gas species is caused by the inner combustion states.