Rapid and Accurate Determination of Bacterial Abundance and their Physiological Activity in Freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth”, Japan 2006-01-2078
In space utilization, an enormous amount of freshwater for drinking, daily use, hydroponics. is used and recycled in a closed habitat. We have developed culture-independent techniques to analyze microbial cells at the single level, a cell itself, and also community level. By using these methods such as fluorescent vital staining (double staining with carboxyfluorescein diacetate and 4′,6-diamidino-2-phenyl indole), microcolony method and denaturing gradient gel electrophoresis (DGGE), bacterial abundance and their physiological activity in freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth” were determined.
Citation: Baba, T., Yamaguchi, N., Nasu, M., Aibe, Y. et al., "Rapid and Accurate Determination of Bacterial Abundance and their Physiological Activity in Freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth”, Japan," SAE Technical Paper 2006-01-2078, 2006, https://doi.org/10.4271/2006-01-2078. Download Citation
