Development and (Evidence for) Destruction of Biofilm with Pseudomonas aeruginosa as Architect 911404
Disinfection and maintenance of an acceptable level of asepsis in spacecraft potable water delivery systems is a formidable task. The major area of research for this project has been to monitor the formation and growth of biofilm, and biofilm attached microorganisms, on stainless steel surfaces (specifically coupons), and the use of ozone for the elimination of these species in a closed loop system.
A number of different techniques have been utilized during the course of a typical run. Scraping and sonication of coupon surfaces with subsequent plating as well as epifluorescence microscopy have been utilized to enumerate biofilm protected Pseudomonas aeruginosa. In addition, scanning electron microscopy is the method of choice to examine the integrity of the biofilm. For ozone determinations, the indigo decolorization spectrophotometric method seems most reliable. Both high- and low-nutrient cultured P. aeruginosa organisms were the target species for the ozone disinfection experiments.
Citation: Uzcategui, V., Donadeo, J., Lombardi, D., Costello, M. et al., "Development and (Evidence for) Destruction of Biofilm with Pseudomonas aeruginosa as Architect," SAE Technical Paper 911404, 1991, https://doi.org/10.4271/911404. Download Citation
Author(s):
Valerie N. Uzcategui, John J. Donadeo, Daniel R. Lombardi, Michael J. Costello, Richard L. Sauer
Affiliated:
Department of Chemistry State University of New York Binghamton, NY
Pages: 18
Event:
International Conference On Environmental Systems
ISSN:
0148-7191
e-ISSN:
2688-3627
Also in:
Spacecraft Water Quality: Maintenance and Monitoring-SP-0874, SAE 1991 Transactions - Aerospace-V100-1
Related Topics:
Water quality
Microscopy
Plating
Steel
Spacecraft
Biological sciences
Reliability
Research and development
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