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Bio-derived butanol is a potential CO2 neutral alternative fuel to be applied in internal combustion engines. However, the physical and combustion characteristics have to be fully understood before it can be used efficiently in engines. This work investigates the viscosity and the combustion characteristics of n-butanol/diesel fuel blends, with a particular focus on the combustion property at low load performance. N-butanol was mixed with diesel from 20 vol% up to 80 vol% blend ratio. Tests were performed in a combustion research unit (constant volume) at fixed chamber pressure that mimic a low load condition. The effects of various chamber temperatures and ambient oxygen concentrations (21%, 13%, and 11%) are evaluated. As expected, the viscosity of n-butanol/diesel fuel blends decreases at high temperature. The decrease is non-linear with the blend ratio.