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An experimental investigation was conducted to determine the efficiency of 1,3-dioxolane as an alternative oxygenate to MTBE in Reformulated Gasoline. In the investigation, the effect of adding 1,3 dioxolane on octane rating was evaluated. The octane number of the fuels was determined using a Waukesha single cylinder, 4-stroke cycle, 2-valve, CFR F-2U octane rating unit. Certified 87 octane gasoline was used as the base fuel which 1,3-dioxolane was added at specific volumetric proportions. Iso-octane (100 octane number) and N-heptane (0 octane number) are primary reference fuels that were blended at volumetric proportions to produce a reference base of known octane number. The reference base fuel of known octane number was used for comparison of knock tendency to the test fuels under the ASTM D 2699 (Research) and ASTM D 2700 (Motor) methods of testing.