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This paper presents calibration results of the two excitation laser wavelength fluorescence of acetone for the determination of temperature fields. The calibration was performed under engine relevant conditions and comprises the simultaneous variation of temperature and pressure in the range of 293 K to 750 K and of 0.1 MPa to 2 MPa, respectively. The influence of different gas compositions, e.g., resulting from exhaust gas recirculation, was checked by calibrating for pure nitrogen, synthetic air and carbon dioxide and for a certain extend for water as bath gas. The two excitation wavelengths used were 248 nm and 308 nm.