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Study of bio-regenerative life support system is a critical issue for long term manned spaceflight applications. An engineering approach of such systems, consists in experimental stoichiometry and growth kinetics determination and building up structured and predictive mathematical models, in order to test simplified biological processes. Up to now, the main results obtained by such an aproach are in good agreement with ground-based data. Despite several scientific experiments performed in space, results on microbial kinetics study remains rare, and the real influence of space environment (micro-gravity, radiation, light spectrum, ..) on micro-organisms growth is still not quantified. Precursor space missions are therefore justified to expand current knowledge and check for the changes affecting such biological processes.