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This paper presents the results of data measurements for a human material balance, which were conducted as part of a 2-day manned test of a regenerative Environmental Control and Life Support System (ECLSS) in an isolation chamber. The manned test was carried out using a man-rated closed chamber operated in airtight mode at the Tsukuba Space Center of NASDA (National Space Development Agency of Japan), Tsukuba, Japan. A five-person Japanese test crew resided inside the isolation chamber for 48 consecutive hours, living and working along a time schedule based upon Space Shuttle and ISS activities. Data measured on human material needs and effluents included drinking water and beverages, food, urine, feces, water vapor (latent respiration and perspiration), and metabolic trace gases, which were measured directly with scales or estimated indirectly in various ways, such as measuring crew weight change, or measuring air temperature and humidity.

A 48-hour manned closed environment test was carried out using the closure environmental adaptation training facility (isolation chamber) at Tsukuba Space Center of NASDA (National Space Development Agency of Japan), in Tsukuba-shi, Ibaraki, Japan. This 48-hour closed test was planned and conducted as a preliminary testing for future manned closed tests of advanced life support systems. The purposes of the test were to obtain basic data related to design requirements of regenerative life support systems, which include environmental data of air, water, waste water, and microorganisms inside the chamber, and data on human mass balance and human consumables during the test. Additionally perform integrated evaluation tests on functions and performance of Environmental Control and Life Support System (ECLSS) subsystem research models which have been fabricated and evaluated so far in independent separate tests.