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Particulate emissions data are presented from a group of 19 in-use diesel automobiles. Five driving cycles and three fuel/lubricating oil combinations have been used to obtain particulate emissions data and also to collect particulate samples for chemical and bioassay characterization studies. Particulate samples were solvent extracted with dichloromethane to obtain the soluble organic fraction (SOF) of the emitted particulate. Particulate and SOF data are presented for emission/distance, emission/time and emission/fuel consumed basis by vehicle manufacture, driving cycle, and fuel/oil subcategory. Some SOF samples have been used in the bioassay test of Ames, and these results are presented for different vehicles and driving cycles. Particulate emissions, SOF emissions and bioassay activity results are presented in terms of mileage accumulation effects.