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Automotive engines and control systems are more and more sophisticated due to increasingly restrictive environmental regulations. Particularly in both diesel and SI lean-burn engines NOx emissions are the key pollutants to deal with and sophisticated Engine Management System (EMS) strategies and after-treatment devices have to be applied. In this context, the in-cylinder oxygen mass fraction plays a key-role due its direct influence on the NOx formation mechanism. Real-time estimation of the intake O₂ charge enhances the NOx prediction during engine transients, suitable for both dynamic adjustments of EMS strategies and management of aftertreatment devices. The paper focuses on the development and experimental validation of a real-time estimator of O₂ concentration in the intake manifold of an automotive common-rail diesel engine, equipped with turbocharger and EGR system.