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Water-emulsified diesel fuel technology has been proven to reduce nitrogen oxides (NOx) and particulate matter (PM) simultaneously at relatively low cost compared to other pollution-reducing strategies. While the mechanisms which result in these reductions have been postulated, the development of new analytical tools to measure in-cylinder soot formation using optically accessible engines can lead to a deeper understanding of combustion and the chemical and physical mechanisms when water is present during combustion. In this study, an optically accessible single cylinder engine was used to study how water brought into the combustion chamber via an emulsified fuel changes the combustion process and thereby reduces emissions. In-cylinder measurements of relative soot concentrations were used to determine the effect of water-emulsified fuel on soot formation.