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The baseline hygiene water reclamation system for Space Station Freedom has been changed from Reverse Osmosis with Multifiltration post-treatment to stand-alone Multifiltration. The Multifiltration concept offers increased system reliability, a decrease in power consumption, and essentially 100% water recovery. Multifiltration is based on well documented sorption technology for removal of contaminant species. System complexity is minimal. Moving parts are limited to one pump and simple valving. Reliable microbial control is obtained by heat sterilization and by the use of iodine as a bactericide. Iodine addition is accomplished in the Unibeds with an iodinated resin which is also used in the Microbial Check Valve (MCV). Microbial Check Valves have proven reliable and effective on board the Space Shuttle since the beginning of the Shuttle program. Power consumption is primarily attributed to heat sterilization. The energy required for the pump and controls is relatively low.

Unibeds®* are multimedia layered sorption beds baselined for use in the Space Station Freedom (SSF) water reclamation system. Unibeds® must be sterilized prior to use to avoid the introduction of bacteria into the water reclamation system when the Unibeds® are routinely changed out. In the past, Unibeds® were autoclaved in an attempt to achieve sterility. Some sorbent media used in the Unibeds® decompose when exposed to high temperatures for extended periods. Although no significant sorbent decomposition occurs during the routine autoclave time of 30 minutes, it is uncertain whether sufficient sterilization temperatures are achieved at the Unibed® core. Gamma irradiation has been evaluated as a practical alternative method to achieve sterility and eliminate possible sorbent thermal degradation. Sorbent media were inoculated with irradiation resistant spores (106 CFU/ML) of bacterium Bacillus pumilus and subsequently exposed to radiation doses of 1.5, 2.0, and 2.5 megarads (Mrad).

On the International Space Station (ISS), humidity condensate will be collected from the atmosphere and treated by multifiltration to produce potable water for use by the crews. Ground-based development tests have demonstrated that multifiltration beds filled with a series of ion-exchange resins and activated carbons can remove many inorganic and organic contaminants effectively from wastewaters. As a precursor to the use of this technology on the ISS, a demonstration of multifiltration treatment under microgravity conditions was undertaken. On the Space Shuttle, humidity condensate from cabin air is recovered in the atmosphere revitalization system, then stored and periodically vented to space vacuum. A Shuttle Condensate Adsorption Device (SCAD) containing sorbent materials similar to those planned for use on the ISS was developed and flown on STS-68 as a continuation of DSO 317, which was flown initially on STS-45 and STS-47.

The ability to aseptically remove samples and products, and the capability for addition of materials to sterile or otherwise microbially susceptible systems have always been compromised by the lack of a reliable means of sterilizing the mating fixtures. Cultures of mammalian cells are particularly vulnerable to microbial contamination due to the complexity of nutrient media and the lengthy periods required for cell growth. The Microwave Sterilizable Access Port has been developed to overcome this limitation. The system consists of three primary components: a microwave power source, a combined sterilization chamber/in-line valve port assembly, and a specimen transfer interface. Microwave energy is transmitted via coaxial cable to a small pressurized chamber that serves as a sterile transition between the surrounding environment and the system during transfer of materials.