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The rising global energy demand, climate change considerations, and worldwide mandates to replace conventional energy supplies with renewable resources continue to drive increased use of biodiesel into the automotive market. OEM's have observed that usage of biodiesel fuel blends can lead to more fuel dilution of engine oil and as a consequence performance issues. The issue is more pronounced in modern, low emissions diesel engines equipped with Diesel Particulate Filters (DPF) in combination with late post-injection of fuel into the cylinders. A prerequisite for the safe use of biodiesel is the availability of a cost effective detection method that allows an accurate measurement of the level of fuel dilution in used engine oil. It contributes to a better fundamental understanding of cause-effect relationships between biodiesel fuel dilution and engine lubricant performance.