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Accident investigators are often required to determine if an occupant was wearing their seat belt during a collision. Previous studies have provided examples of physical evidence relied upon by investigators to determine if the seat belt assembly was subjected to occupant restraint loading. This paper examines the potential for clothing fibers to be permanently transferred to the seat belt webbing during a collision. To evaluate fabric transfer evidence as an indicator of restraint usage by an occupant during a collision, the following issues were examined: automotive seat belt webbing construction and behavior under load, fiber evidence found on the webbing in new and used conditions, and the transfer of different types of clothing fibers to webbing during full-scale sled testing.