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For flows around a tire rotating over a ground plane, the Reynolds number is probably the most important parameter influencing the transition mechanism leading to flow separation from the tire surface, as it determines the viscous response of the boundary layer in the vortex-wall interaction. The present work investigates the effects of Reynolds number on an isolated tire model using a commercial Computational Fluid Dynamics (CFD) code. It validates the baseline simulation for this purpose against the Particle Image Velocimetry (PIV) data from Stanford University got using a Toyota Formula 1 race car tire model. Time-resolved velocity fields and vortex structures from the PIV data are used to correlate local and global flow phenomena to identify unsteady boundary-layer separation and the subsequent flow structures. The study will highlight the pre to post critical flow regimes where the aero coefficients and vortex structure will be studied.