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Valve deactivation followed by multiple compression-expansion strokes was employed to remove intake-generated turbulence from the bulk gas in an internal combustion engine. The result was a nearly quiescent flowfield that retains the same time-varying geometry and, to a first approximation, thermodynamic conditions as a standard engine. Mass loss, and more significantly heat loss were found to contribute to a reduction in the peak cylinder pressure in the cycle following two compression-expansion strokes. The reduction of the turbulence was verified both computationally and by performing premixed combustion studies. Mixing studies of both liquid spray jets and gaseous jets were performed. Laser-induced fluorescence images of high spatial resolution and signal-to-noise ratio were acquired, allowing the calculation of the two in-plane components of the scalar dissipation rate.