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Many incidents associated with filter plugging have extensively been reported in microbially contaminated diesel and biodiesel fuel systems, especially under long term storage conditions. In this study a quantitative assessment of the undesirable insoluble solids produced in contaminated biodiesel fuels was carried out in order to evaluate their evolution rate during biodeterioration. For this purpose, a series of contaminated biodiesel fuel microcosms were prepared and stored for six months under stable conditions. The quantity of the particulate contaminants was monitored during storage by a multiple filtration technique which was followed at the end by a comparison with the active bioburden per ATP bioluminescence protocol. Additionally, identical microcosms were treated with a commercially available biocide in order to examine the latter’s activity both on solids formation and the microbial proliferation.

Biofuels are a promising alternative to fossil fuels as their availability has been reduced during the last decades and they are the main sources of greenhouse gases emissions. Moreover, the targets of the international regulations include reduction of fossil fuels consumption, and improvement of the sustainability of the vehicle fleet. Blending gasoline with biofuels will result in changes in fuel blending procedures and combustion process especially for the gasoline direct injection (GDI) engines. In this article, flame visualization using chemiluminescence techniques in a Single Cylinder Optical Research Engine (SCORE) is presented, with an adjusted intake pressure of 850 mbar and early intake single injection (280 CAD BTDC), by using 100% hydrocarbon-based gasoline, E10 (90% gasoline - 10% ethanol) and ETBE20 (80% gasoline - 20% ethyl tert-butyl ether). ETBE20 is a potential alternative for E10, as it contains the same amount of renewable fuel and has low water solubility.