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In the frame of circular economy, wastes are perceived as sources not only for the recovery of high added-value compounds but also for energy production. Coffee is one of the most popular beverages with the consumption continuously increasing and generating huge amounts of solid residues in return. This solid waste after the extraction of the coffee beverage is known as Waste or Spent Coffee Grounds (WCG). Among others, the valorization has the potential to be directed as a bioresource for sustainable energy and particularly for the production of alternative liquid fuels for internal combustion engines. The aim of the current study is to formulate alternative fuel from WCG and to examine the fundamental properties per relevant specifications and requirements. Parameters related to stability, cold flow properties, lubricating characteristics and ignition quality are studied in comparison with other types of biodiesel fuel.

Many incidents associated with filter plugging have extensively been reported in microbially contaminated diesel and biodiesel fuel systems, especially under long term storage conditions. In this study a quantitative assessment of the undesirable insoluble solids produced in contaminated biodiesel fuels was carried out in order to evaluate their evolution rate during biodeterioration. For this purpose, a series of contaminated biodiesel fuel microcosms were prepared and stored for six months under stable conditions. The quantity of the particulate contaminants was monitored during storage by a multiple filtration technique which was followed at the end by a comparison with the active bioburden per ATP bioluminescence protocol. Additionally, identical microcosms were treated with a commercially available biocide in order to examine the latter’s activity both on solids formation and the microbial proliferation.

The diesel fuel supply chain faces new challenges associated with microbial contamination symptoms in biodiesel fuel. FAME's (Fatty Acid Methyl Esters) chemical composition along with its hygroscopic nature makes it more “biologically active” and as a result the final blends could be more prone to microbiological contamination. Survey of in-field incidents and facts in the Greek supply chain indicate that biodiesel is more prone to microbial growth. Furthermore, several experimental studies which demonstrate the susceptibility of biodiesel fuel for microbial growth have been conducted in the laboratory. The influence of FAME has been evaluated as well as the effect of microbial proliferation on the quality of the blend. Different types of biodiesel have been blended with Ultra Low Sulphur Diesel at various concentrations, and the resulting blends were mixed with bottom-water of known viable microbial colonies and stored.

The fuel supply chain faces challenges associated with microbial contamination symptoms. Microbial growth is an issue usually known to be associated with middle distillate fuels and biodiesel, however, incidents where microbial populations have been isolated from unleaded gasoline storage tanks have also been recently reported. Alcohols are employed as gasoline components and the use of these oxygenates is rising, especially ethanol, which can be a renewable alternative to gasoline, as well. Despite their alleged disinfectant properties, a number of field observations suggests that biodeterioration could be a potential issue in fuel systems handling ethanol-blended gasoline. For this reason, in this study, the effect of alcohols on microbial proliferation in unleaded gasoline fuel was assessed. Ethanol (EtOH), iso-propyl alcohol (IPA) and tert-butyl-alcohol (TBA) were evaluated as examples of alcohols utilized in gasoline as oxygenates.

The aim of this study was to investigate the effect of a variety of phenolic type antioxidant additives on the microbial stability of biodiesel and diesel/biodiesel blends. Six synthetic phenolic type antioxidant agents were added in FAME at concentrations up to 1000 ppm. Treated FAME was also blended with Ultra Low Sulfur Diesel (ULSD) fuel at a concentration of 7% v/v in order to examine the activity of the substances in the final blends. The oxidation stability in the presence of the phenolic compounds was determined by carrying out measurements under accelerated oxidation process in the Rancimat unit. The effectiveness of those antioxidant agents against microbial contamination in biodiesel fuel was studied under certain testing protocols for detecting microbiological activity in the fuel supply chain and for evaluating antimicrobials against fuel bio-deterioration.