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Part substitution of alcohol in gasoline alters the composition and combustion characteristics leading to different particulates as emitted by individual fuel. This paper presents the test work done on characterisation of particulate number concentration emitted from four stroke engine powered two wheeler with neat Euro III gasoline and its blend with ethanol (5, 10, 20 & 30 %) using Electrical Low Pressure Impactor (ELPI). The characterisation of particulates was carried out under different operating conditions viz. Indian Driving Cycle (IDC), Road Load Simulation (RLS) and Wide Open Throttle (WOT) conditions on Chassis Dynamometer with the test fuels. It was observed from the study that for all the test fuels, the number concentration increases as the particulate size reduces. Further, it was observed that addition of ethanol in neat Euro III gasoline has reduced the particulate number concentration both in transient and steady speed conditions.