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Discomfort glare while driving at night might have implications for long-term fatigue and ultimately, driving performance and safety. The intensity of oncoming headlights, their spectral power distribution, the location of the lights in the field of view, and the ambient illumination conditions can all impact feelings of discomfort while driving at night. Not surprisingly, light sources with higher intensities are perceived as more glaring. Similarly, perceptions of discomfort increase as the ambient lighting conditions are reduced, and as the glare sources are located closer to the line of sight. Recent research also appears to demonstrate the role of short-wavelength light in contributing to the discomfort glare response. The present paper outlines a laboratory study to probe the effects of ambient light level, spectral power distribution, and control of gaze on discomfort glare, and potential interactions among these factors.