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The reduction of carbon footprint of compression ignition engines for road transport makes it necessary to search for clean fuels alternative to diesel and to evaluate them under engine conditions. For this reason, in this paper, the combustion behaviour of different blends of synthetic fuels has been analyzed in an optical single cylinder engine of Medium Duty size (0,8 liters per cylinder) by means of optical techniques. The aim is to evaluate the effect of synthetic fuels, both partly or completely fossil diesel, in terms of combustion behaviours and soot formation. Therefore, different blends of oxymethylene dimethyl ether (OMEX) with diesel and neat hydrotreated vegetable oil (HVO) were studied. A conventional common rail injection system and a single injection strategy was used. In addition, special care was taken to ensure that conditions inside the engine cylinder at the injection start were as close as possible to the conditions used in previous studies.