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The use of portable emissions measurement systems (PEMS) for testing vehicle emissions while driving on the road has been demonstrated as early as the 1980s. Many users have taken the driving route and repeated the route in a chassis cell with the same vehicle expecting identical results. Emission results can be comparable but there are many factors that need to be considered. This study compares PEMS results for a driving route repeated across seasons and traffic conditions with a single vehicle. The ambient temperature variability and traffic is shown to cause variation in emissions for any individual run. Generating a test cycle to mimic the driving route can be done in a variety of ways. The simplest is to take an individual driving run and translate the time and speed trace directly. This does not address the statistical results from numerous driving runs on the same route.