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This paper investigates how particulates number PN is influenced by fuel wall-film, liner wetting, and the mixing quality for different start of injection timings (SOI). Both experimental data with PN measurements, endoscope images from a high-speed camera from a single-cylinder engine, and CFD simulations were used for the analysis. Engine geometry was a spray-guided system with 300 bar fuel pressure and with single injections. Data was captured for 2000 rpm / 9 bar IMEPn. The results show that fuel film on the piston was only found to significantly increase PN for over-advanced SOI (in our engine geometry, earlier than -310 CAD). This results in luminescence from diffusion burn on the piston surface, which strongly contributes to PN. For an SOI timing of -310 CAD, fuel film on piston reaches a maximum of 3% of the injected fuel, vaporizes, and no remaining fuel film is found at the time of ignition. Approximately 0.5-1% of the fuel ends up on the liner.