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Widespread use of biofuels for automobiles would greatly reduce CO2 emissions and increase resource recycling, contributing to global environmental conservation. In fact, activities for expanding the production and utilization of biofuels are already proceeding throughout the world. For diesel vehicles, generally, fatty acid methyl ester (FAME) made from vegetable oils is used as a biodiesel. In recent years, hydrotreated vegetable oil (HVO) has also become increasingly popular. In addition, biomass to liquid (BTL) fuel, which can be made from any kinds of biomass by gasification and Fischer-Tropsch process, is expected to be commercialized in the future. On the other hand, emission regulations in each country have been tightened year by year. In accordance with this, diesel engines have complied with the regulations with advanced technologies such as common-rail fuel injection system, high pressure turbocharger, EGR and aftertreatment system.