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To comply with the ambitious CO2 targets of the European Union, greenhouse gas emissions from the transport sector should be eliminated by 2050. Incremental powertrain improvement and electrification are only a part of the solution and need to be supplemented by carbon-neutral fuels. Due to the high technology readiness level, biofuels offer a short-term decarbonization measure. The high process energy demand for transesterification or hydrotreating however, hinders the well-to-wheel CO2 reduction potential of current market biodiesels. An often-raised, economically and energetically feasible alternative is to use unprocessed oils with viscosity and cold-properties improvers instead. The present work investigates the suitability of one such biofuel (PlantanolTM) for advanced common rail engines operating in a partially premixed compression ignition mode. Preliminary investigations are carried out on a Euro VIb light-duty car engine.