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The aim of this work has been to investigate the influence of bio-diesel (FAME - fatty acid methyl ester) and reasonable blends of FAME and diesel, on the behavior of the mixture formation, the combustion process and the emission levels of a modern diesel engine. Therefore experimental investigations have been carried out on a single cylinder engine which is a modification of a four cylinder production HSDI (high speed direct injection) diesel engine. Cylinder pressure and tail-pipe emissions have been measured. Additionally the injector has been mounted on a flow-metering device to gather information about the pure flow through the injection system depending on the fuel type. In parallel with the experimental investigations CFD (computational fluid dynamics) calculations have been carried out in order to closely check local effects inside the injector and the combustion chamber.