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The higher portion of biodiesel blended fuel will result in lower power output since biodiesel itself has lower energy input (B20 gave about 3% lower torque output at peak torque speed). In the ELR (Engine Load Response) Test Cycle, biodiesel blended fuel emitted less smoke than diesel fuel, while CO and NOx emission of biodiesel blended fuel and diesel fuel are comparable. Biodiesel particulate matter (PM) seemed to be higher than diesel fuel. In addition, additized biodiesel blended fuels (B5, B10 and B20) proved qualitative in oxidation stability, acid value, etc. Biodiesel specific lubricant confirmed its functions by evaluating the viscosity increase, fuel dilution, TAN, TBN and wear metal content during engine durability test. The used oil analysis affirmed that the lubricant could effectively be used with particular biodiesel blended fuel in advanced, heavy-duty common-rail DI diesel engines.