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In order to investigate the impact of the Ethanol content on existing domestic Gasoline vehicles, we conducted an exhaust gases test, a fuel evaporative emissions test, a high-temperature driveability test, and a material impact test. As a result, no safety problems occurred in the metal material impact test at an Ethanol content of 3% or less. In the exhaust gases test, the fuel evaporative emissions test, and the high-temperature driveability test, no problems occurred at an Ethanol content of 3% or less. Based on these results and discussions conducted by the fuel policy subcommittee of the advisory committee for natural resources and energy survey, it was concluded that the Ethanol content in Gasoline must be 3% or less and the oxygenate (alcohol etc.) content must be limited to a value corresponding to a total oxygen content of 1.3% or less. The results obtained by this study were reflected in the Japanese Gasoline compulsory quality regulations.