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Compared with diesel fuel, FAME is relatively unstable and readily generates acids such as acetic acid and propionic acid. When FAME-blended diesel fuel is used in existing diesel vehicles, it is important to maintain the concentration of FAME-origin acid in the fuel at an appropriately low level to assure vehicle safety. In the present study, the oxidation of diesel fuel containing 5% FAME is investigated. Several kinds of FAMEs were examined, including reagents such as methyl linoleate and methyl linolenate, as well as commercially available products. The level of acid, peroxide, water, and methanol and the pressure of the testing vessel were measured. The result shows that unsaturated FAMEs that have two or more double bonds are unstable. Also, water is generated by oxidation of FAME blended diesel fuel, accelerating corrosion of the terne sheet.

In order to investigate the impact of the Ethanol content on existing domestic Gasoline vehicles, we conducted an exhaust gases test, a fuel evaporative emissions test, a high-temperature driveability test, and a material impact test. As a result, no safety problems occurred in the metal material impact test at an Ethanol content of 3% or less. In the exhaust gases test, the fuel evaporative emissions test, and the high-temperature driveability test, no problems occurred at an Ethanol content of 3% or less. Based on these results and discussions conducted by the fuel policy subcommittee of the advisory committee for natural resources and energy survey, it was concluded that the Ethanol content in Gasoline must be 3% or less and the oxygenate (alcohol etc.) content must be limited to a value corresponding to a total oxygen content of 1.3% or less. The results obtained by this study were reflected in the Japanese Gasoline compulsory quality regulations.