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Many efforts have been made in recent years to find renewable replacements for fossil fuels that can reduce the carbon footprint without compromising combustion performance. Bio-blendstock oil developed from woody biomass using a reliable thermochemical conversion method known as catalytic fast pyrolysis (CFP), along with hydrotreating upgrading has the potential to deliver on this renewable promise. To further our understanding of naphthenic-rich bio-blendstock oils, an improved formulation surrogate fuel (SF), SF1.01, featuring decalin and butylcyclohexane naphthenic content was devised and blended with research-grade No.2 diesel (DF2) at various volume percentages. The blends were experimentally evaluated in a single-cylinder Ricardo Hydra compression ignition engine to quantify engine and emissions performance of SF1.01/DF2 blends. Injection timing events were varied from knock limit to misfire limit at the same operating conditions for all blends.